Allergy · ALEX3 Testing

What Does ALEX3 Test For?

Dr Raj Dhillon  ·  September 2026  ·  5 min read

ALEX3 (Allergy Explorer 3) is a multiplex allergy immunoassay that measures specific IgE antibodies to over 300 allergens and allergen components from a single blood draw. It's the most comprehensive allergy blood test currently available in routine clinical practice.

Allergen Categories Covered

Tree Pollens

Birch, alder, hazel, oak, ash, plane, cedar, olive, and all UK-relevant tree species. Molecular components included.

Grass Pollens

Timothy, rye grass, Bermuda grass, and other species — including component testing (Phl p 1, Phl p 5, Phl p 7, Phl p 12).

Weed Pollens

Mugwort, ragweed, plantain, nettle — all seasonal UK and European weed pollens.

House Dust Mites

Dermatophagoides pteronyssinus and D. farinae (the two most common species) plus mite component allergens (Der p 1, Der p 2, Der p 23).

Animal Danders

Cat, dog, horse, rabbit, guinea pig, rat, mouse — plus species-specific molecular components (e.g., Fel d 1 for cat).

Moulds

Alternaria, Cladosporium, Aspergillus, Penicillium — the four most clinically significant moulds in UK environments.

Food Allergens

Peanut, tree nuts (cashew, walnut, hazelnut, almond, pecan, pistachio), milk, egg, wheat, soya, shellfish, fish, sesame, and more — with molecular components.

Insect Venoms

Honeybee (Api m 1) and wasp/yellow jacket (Ves v 5) — including cross-reactive carbohydrate determinants for accurate interpretation.

Molecular Components

PR-10 proteins, lipid transfer proteins, profilins — the cross-reactive components that explain pollen-food syndrome and other cross-reactions.

Why Molecular Components Matter

Traditional allergy tests measure IgE to whole allergen extracts. ALEX3 adds a further layer by identifying sensitisation to individual proteins within those extracts. This matters clinically for two reasons:

ALEX3 is not a food intolerance test and does not measure IgG antibodies. It measures IgE — the immune mechanism behind true (type 1) allergic reactions. Food intolerances are a separate phenomenon not detected by this test.

What the Results Look Like

Results are returned as a detailed allergen profile — each allergen or component is scored by class (0–6) based on the specific IgE level in kU/L. Your GP uses this alongside your clinical history to determine which results are clinically significant, which may represent cross-reactions, and what management is appropriate.

Common Questions

Do I need to stop antihistamines before the test?

No. ALEX3 is a blood test and is unaffected by antihistamine use. Take your regular medication as normal.

Can children have ALEX3 testing?

Yes. ALEX3 is suitable from infancy onwards. The single blood draw is often preferred for young children over multiple skin prick tests.

Can I have ALEX3 if I have eczema?

Yes — and it's particularly useful. Active eczema can make skin prick testing unreliable; the blood test bypasses this entirely.

How quickly are results returned?

Typically 3–5 working days. Your results are reviewed by Dr Raj Dhillon and discussed at a follow-up appointment or via a written summary.

Book ALEX3 Testing at Lambert Medical Practice

The most comprehensive allergy blood test available — 300+ allergens, molecular component analysis, results within days.

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